Describir un método rápido, sensible y específico para la identificación de A. Los brotes nosocomiales de aspergilosis son cada vez más frecuentes, pero su identificación y caracterización epidemiológica es lenta y laboriosa. Aspergillus fumigatus es un hongo filamentoso que se comporta como patógeno oportunista y constituye una de las complicaciones infecciosas más importantes en los pacientes inmunocomprometidos. Touchdown PCR combined with RAPD analysis is a sensitive, accurate method for managing clinical outbreaks of Aspergillus fumigatus. Patterns may be easier to repeat and interpret when longer ramp times are used. RAPD analysis is a fast, reliable tool for DNA fingerprinting. Best RAPD results were obtained with the R-108 primer and considerably longer ramp times between annealing and extension. Touchdown PCR and phenotype results were identical. Genotyping was performed with random amplification of polymorphic DNA (RAPD) analysis, comparing the results from two primers (OPZ-19 and R-108) and different amplification protocols with regard to band resolution and reproducibility. fumigatus was identified by both touchdown PCR and conventional phenotyping methods. fumigatus isolated from patients with invasive aspergillosis (n 5 4), the hospital environment (n 5 5) and reference cultures (n 5 1), as well as other close phylogenetic fungal species from patients (n 5 1), hospital environment (n 5 6) and reference cultures (n 5 1). fumigatus using methodology available in clinical laboratories. Description of a fast, sensitive, specific method to identify and fingerprint A. Nosocomial outbreaks of aspergillosis are becoming more frequent, but their identification and epidemiological characterization is slow and difficult.
Aspergillus fumigatus is a filamentous fungus that acts as an opportunistic pathogen and has emerged as a major problem in immunosuppressed patients.
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